Download Indigo Carmine Color Change Images. Indigo carmine is an organic sodium salt resulting from the formal condensation of indigo carmine (acid form) with two equivalents of sodium hydroxide. The surgery ended approximately 3.5 hour after the first dye injection.
The indigo carmine was administered via the left side iv line and 5 minutes later the dye was confirmed at the surgical field.
Shaking the flask brings more oxygen into the solution, provoking the formation of the oxidized (green) form. Which results in a color change. Indigo carmine undergoes a reversible reduction. Prepare a 750 ml aqueous solution with 15 g glucose (solution a) and a 250 ml aqueous solution with 7.5 g sodium hydroxide (solution b).